Fitc painting assay
WebStaining. 1. Wash cells twice with cold PBS and then resuspend cells in 1X Binding Buffer at a concentration of 1 x 10^6 cells/ml. 2. Transfer 100 µl of the solution (1 x 10^5 cells) to a … WebMar 5, 2024 · FITC painting assay. WT and NIK LEC-KO mice were shaved and painted on the posterior flank skin over inguinal LNs with 10 μl of 1% fluorescein isothiocyanate (FITC) ...
Fitc painting assay
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WebAbout This Assay Cayman’s Annexin V FITC Assay Kit employs a FITC-conjugated annexin V as a probe for phosphatidylserine on the outer membrane of apoptotic cells. Propidium iodide (PI) is used as a marker of cell membrane permeability (see Figure 1 on page 7). This kit is valuable for researchers looking to assess the WebOct 22, 2024 · For this purpose, we performed an FITC painting assay, in which FITC is applied epicutaneously of one flank of the mouse and allows measuring migratory immune cell properties into the draining lymph node …
WebDec 15, 2024 · FITC painting assay. We shaved and depilated mice 2 d before the assay. We painted mice on four spots of the flank back skin (∼5 mm in diameter per spot) with 10 μl of a 0.5% FITC solution (Isomer I; Millipore Sigma) 4 h prior to the assay as previously described (20, 23). The FITC solution was prepared in acetone:dibutyl phthalate (1:1, vol ... WebThe Cell Painting assay, in A549 and U2OS cells. ... 1 Alternately, a FITC (482/536) filter may be used. 2 The reagent SYTO 14 was selected after comparing a number of RNA …
WebFITC Painting Assay. To assess the migratory capacity of CD11b+ cells, including monocytes/macrophages, rat flank was shaved and painted with 300 μl of 5 mg/ml FITC dissolved in the diluent composed of equal volume parts of acetone and dibutylphthalate . After 24 h, individual lymph node cell suspensions were prepared of pooled inguinal ... WebNov 28, 2013 · One of three representative experiments is shown. (b) FITC painting assay with gating on FITC+ migratory DC versus total migratory DCs in Flt3L-treated (upper) and control mice (lower). CD11b- migDCs (red) directly capture FITC in the skin and traffic to the skin-draining LNs at 16 hours and 24 hours in C57BL6/WT mice ...
WebJul 9, 2014 · A: Lung permeability assay using 10 mg of FITC-Dextran kg b.w. via i.n. instillation in the ALI induced by different doses (0.5, 2 and 4 mg/kg b.w.) of LPS by i.n. instillation (n = 3). The fluorescence intensity …
WebAnnexin V-FITC Microscopy Staining Protocol. 1. Wash cells twice with 1X PBS. 2. Wash cells once with 1X Annexin V Binding Buffer. 3. Stain cells with Annexin V-FITC diluted 1:10 in 1X Annexin V Binding Buffer for 15 min at RT. We typically use 2 ml total volume per Labtek II chamber slide (i.e., 200 µl Annexin V-FITC and 1.8 ml 1X Binding Buffer. small plastic boxes with lids suppliersWebNational Center for Biotechnology Information small plastic buckets ukWebChoosing DNCB or FITC-induced Contact Hypersensitivity Models. Posted by MD Biosciences. on Jun 1, 2011 10:37:00 AM. Contact hypersensitivity dermatitis occurs … highlights asia cup 2018small plastic box display caseWebThe protocol suggests 40-80 µg per mg of antibody; for a first-time titration of FITC, try a range of 10 to 400 µg FITC per mg of antibody (for instance, 10, 40, 80, 160, 320 µg per … highlights astros game last nightWebPohang University of Science and Technology. Based on my experience, I only answer questions (1) and (2). (1) Permeability was measured using a 1 mg/mL FITC-dextran … highlights ashes testWebProduct Overview. Recommendations. Documents. FTC-Casein, 2.5 mg, lyophilized, FRET/FP Certified Grade, for use with the Pierce Fluorescent Quantitative Protease … highlights astros